Scoville scale & measurements

[Arcane]

It seems like I'll be having the possibility to do some capsaicin concentration measurements on capsicum. It would make a marvellous topic for my EE (Extended Essay). Has anyone here any experience on doing such measurements? I could also use some information on usage of the HPLC device.

I know that  the HPLC is used, but that's about that. How is the device to be adjusted? How to make the standard solutions? As far as I have understood, the standard solutions require pure capsaicin? Where to get such thing?

[Arcane]

I'm not planning to buy Blair's stuff for this, it's way too expensive and there's too much of capsaicin. I found a company that sells chemical substances, they have also capsaicin.